Green Synthesis of Ag/Fe3O4 Nanocomposite by Aqueous Extract of Eryn-gium Caucasicum and Evaluation of its’ Antifungal Activity

Background and Objectives: The green nanoparticle synthesis method using plants due to a cost effec�tive and environmental compatibility has been extensively noticed. In this study, the biosynthesis of Ag/Fe3O4 nanocomposite by using extract of Eryngium caucasicum and its antifungal properties has been reported. Methods: Synthesized Ag/Fe3O4 nanocomposite was characterized by using UV-Visible spectroscopy, XRD, and FESEM analyses. The antifungal potential of Ag/Fe3O4 nanocomposite was evaluated using Disc diffusion assay. Results: The formation of Ag/Fe3O4 nanocomposite has confirmed through UV–visible spectroscopy (at 425 nm). XRD results revealed the crystalline nature of Ag/Fe3O4 nanocomposite and FESEM analysis exhibited spherical shape with size 26-42 nm. It was also detected that the Ag/Fe3O4 nanocomposite at a concentration of 150μg/ml had the highest inhibition on Cryptococcus neoformans compared to Fusarium thapsinum, Candida albicans, Pyricularia oryzae, Fusarium semitectum and Candida glabrata. Conclusion: The results of this study showed that the Green synthesis of Ag/Fe3O4 nanocomposite by aqueous extract of Eryngium caucasicum, which is a new, non-toxic, simple, cost-effective, fast and envi�ronmentally friendly technique, have high antifungal activity.